S
choller
et al.: Erysiphe platani – monitoring of an epidemic spread in Germany
267
U. B
raun
AB292726, E. hypophylla (N
evod
.) U.
B
raun
& C
unningt
. AB257431, E. juglandis (G
olo
vin
) U. B
raun
& S. T
akam
. AB015928, E. katumo-
toi (U. B
raun
) U. B
raun
& S. T
akam
. AB015917, E.
ligustri (H
omma
) U. B
raun
& S. T
akam
. AB295457,
E. liriodendri S
chwein
. AF011302, E. macleayae
R. Y. Z
heng
& G. Q. C
hen
AB016048, E. magni-
fica (U. B
raun
) U. B
raun
& S. T
akam
. GU195045,
E. monascigera S
hiroya
, C. N
akash
. & S. T
akam
.
AB331646, E. multappendicis (Z.Y. Z
hao
& Y.N.
Y
u
) U. B
raun
& S. T
akam
. AB104520, E. necator
S
chwein
. EF080860, E. nomurae (U. B
raun
) U.
B
raun
AB331648, E. ornata (U. B
raun
) U. B
raun
& S. T
akam
. var. ornata HM057441, E. paeoniae
R. Y. Z
heng
& G. Q. C
hen
AB257436, E. palczew-
skii (J
acz
.) U. B
raun
& S. T
akam
. GQ497277, E.
pisi DC. GU361635, E. platani (H
owe
) U. B
raun
& S. T
akam
. AF011311, AF073349, E. polygoni
DC. AF011308, E. prunastri DC. AB046983, E.
pseudocarpinicola (Y. N
omura
& T
anda
) U. B
raun
& S. T
akam
. AB252466, E. pseudolonicerae (E. S.
S
almon
) U. B
raun
& S.T
akam
. AB015915, E. pulch-
ra var. japonica
(P. H
enn
) U. B
raun
AB000941,
E. quercicola S. T
akam
. & U. B
raun
HQ328834,
E. staphyleae (S
awada
) U. B
raun
& S. T
akam
.
AB015922, E. symphoricarpi (H
owe
) U. B
raun
& S. T
akam
. AY322494, E. syringae S
chwein
.
DQ184478, E. togashiana (U. B
raun
) U. B
raun
& S. T
akam
. AB091775, E. trifoliorum (W
allr
.) U.
B
raun
GU361633, E. trinae H
arkn
. AB022351;
E. urticae (W
allr
.) S. B
lumer
AB104524, E. van-
bruntiana var. sambuci-racemosae (U. B
raun
) U.
B
raun
& S. T
akam
. AB015925, E. wallrothii (U.
B
raun
) U. B
raun
& S. T
akam
. AB015930, E. wei-
geliae Z. X. C
hen
& S. B. L
uo
AB015931.
To elucidate the phylogenetic position of Erysi
phe platani ITS sequences were analysed within
a dataset covering all Erysiphe species of which
sequences were available in GenBank.
Sequence alignment was obtained using MAFFT
6.853 (K
atoh
et al. 2002, 2005, K
atoh
& T
oh
2008)
using the L-INS-i option. To obtain reproducible
results, manipulation of the alignment by hand as
well as manual exclusion of ambiguous sites were
avoided as suggested by G
iribet
&W
heeler
(1999)
and G
atesy
et al. (1993), respectively. Highly diver-
gent portions of the alignment were omitted using
GBlocks 0.91b (C
astresana
2000) with the follow-
ing options: ‘Minimum Number of Sequences for
a Conserved Position’ to 34, ‘Minimum Number of
Sequences for a Flank Position’ to 34, ‘Maximum
Number of Contiguous Non-conserved Positions’
to 8, ‘Minimum Length of a Block’ to 5 and ‘Allowed
Gap Positions’ to ‘With half’.
The resulting alignment [new number of positions:
526 (31% of the original 1645 positions) number
of variable sites: 213] was used for phylogenetic
analyses using a Bayesian Approach (BA) and
Maximum Likelihood (ML). For BA a phylogenet-
ic inference using a Markov chain Monte Carlo
technique was used as implemented in the com-
puter program MrBayes 3.1.2 (H
uelsenbeck
&
R
onquist
2001, R
onquist
& H
uelsenbeck
2003).
Four incrementally heated simultaneous Markov
chains were run over 5 000 000 generations us-
ing the general time-reversible model of DNA
substitution with gamma-distributed substitution
rates and estimation of invariant sites, random
Table 1. List of Erysiphe platani specimens examined in the course of this study with host plants, GenBank acces-
sion numbers, and reference specimens.
Platanus
GenBank acc. no.
Reference specimens
1
Host
(ITS/LSU)
P. acerifolia JQ365940/JQ365936 Germany, Baden-Württemberg, Tübingen,
(A
iton
) W
illd
.
Herrenberger Straße, 21.08.2010, leg. M. L
utz
, KR26134.
P. acerifolia JQ365941/JQ365937 Italy, Toscana, Livorno, Isola d‘Elba, Capoliveri, 30.06.2011,
leg. M. L
utz
, KR27975
P. acerifolia JQ365942/JQ365938 Germany, Baden-Württemberg, Denzlingen, 22.10.2007,
leg. B. M
etzler
, KR26434
P. orientalis L. JQ365943/JQ365939 Greece, Évia, Karystos, Palaia Styra (Old Styra), 24.08.2011,
leg. M. S
choller
, KR29265
1
KR – Fungus collections of the herbarium of Staatliches Museum für Naturkunde, Karlsruhe, Germany.
